CytogenLympho

Special medium for the cultivation of peripheral blood lymphocytes for chromosome evaluation with phytohaemagglutinin (PHA) as mitogen, fetal bovine serum (FBS), L-glutamine and Pen/Strep as antibiotic.

 

Unit: 100 ml

Item number: LGM-100 Category:

Composition

The CytogenLympho medium is supplied deep-frozen and ready to use. It contains basal medium, pre-tested fetal bovine serum (FBS), phytohaemagglutinin as mitogen, L-glutamine, penicillin/streptomycin as antibiotic and phenol red as pH indicator. Thanks to the homogeneous "ready to use" formulation, technical errors and contamination risks in the culture are minimized.

Features and benefits

  • Ready-to-use medium with tested FBS
  • Suitable for heparin blood and lymphocyte-enriched blood
  • More growth through optimized PHA (fraction + concentration)
  • For in-vitro diagnostics (CE IVD)

CE marking means that our product fulfills the requirements of the European Commission Directive 98/79/EC.

However, each user must check (validate) the medium in their own environment before using it in the diagnostic routine to ensure that it is suitable under the specific local conditions.

Analysis and specificity

  • pH value between 7.0 and 7.5
  • Osmolality from 280 to 340 mOsmol / kg
  • Endotoxin <25 EU / ml
  • Mycoplasma negative
  • Sterility: Germs negative
  • Shelf life: 15 months from date of manufacture

CytogenLympho can also be used for genotoxic testing of drug impurities and new active substances in the preclinical phase according to the following OECD guidelines.

  • OECD 487 In Vitro Mammalian Cell Micronucleus Test from 29. Jul. 2016
  • OECD 473 In Vitro Mammalian Chromosomal Aberration Test from 29. Jul. 2016

Transportation and storage

Frozen ≤ -18°C